While the “spatial transcriptomics” emerges as a powerful tool to interrogate the RNA expression of a biological specimen with spatial locations, it remains an assembling measurement that elaborates the collective expression of RNAs in a group of cells (~10-100). I have proposed a new concept of “Single Molecule Spatial Transcriptomics” to interrogate transcriptomes in single cells at the single molecule level. We focus on the physical interpretation of the complexity and dynamics of RNAs in the biological system.
Bio-Question 3: Any physics principles for the sub-cellular RNA distribution?
Related Publications
- T. Wu, F. Syed, E. Simpson, C.-C. Lee, J. Liu, G. Changb, C. P. Dong, C. Seitzb, D. L. Eizirik, R. G. Mirmira, Y. L. Liu, and C. Evans-Molina, “The Impact of Pro-Inflammatory Cytokines on Alternative Splicing Patterns in Human Islets” Diabetes, 71, 116 (2022). (Cover article)
- Cui, J. Liu, and J. Irudayaraj, “Beyond Quantification: In situ Analysis of Transcriptome and Pre-mRNA Alternative Splicing at the Nanoscale”. WIREs Nanomedicine & Nanobiotechnology, 9, e1443 (2017). (Cover article)
- Liu, and J. Irudayaraj, “Non-fluorescent quantification of single mRNA with transient absorption microscopy”, Nanoscale, 8, 19242-19248 (2016). (Cover article)
- Cui, X. Wang, W. Ren, J. Liu, and J. Irudayaraj, “Optical Clearing Delivers Ultrasensitive Hyperspectral Dark-Field Imaging for Single-Cell Evaluation”, ACS Nano, 10, 3132-3143 (2016).
- Liu, I. Cho, N. Damayanti, and J. Irudayaraj, “Single Molecule Detection in Cancer Tissues using Second Harmonic Generation Nanocrystals”, Journal of Biomedical Optics 20, 096016 (2015).